Temperature-sensitive Hydrogenase and Hydrogenase Synthesis in a Psychrophilic Bacterium.

Upadhyay, J. (Washington State University, Pullman) and J. L. Stokes. Temperature-sensitive hydrogenase and hydrogenase synthesis in a psychrophilic bacterium. J. Bacteriol. 86:992-998. 1963.-Hydrogenase and its synthesis were more heat-sensitive in psychrophilic strain 82 than in mesophilic Escherichia coli. The enzyme was not formed above 20 C by the psychrophile, whereas it was formed by E. coli and other mesophiles at 45 C. Aerobically grown cells of strain 82 do not contain hydrogenase but could be induced to form the enzyme by incubation with glucose and amino acids. Hydrogenase adaptation proceeded best at pH 8.0. The psychrophile hydrogenase was destroyed 50% by exposure to 60 C for 2 hr compared with 25% destruction of mesophile hydrogenase under the same conditions. The psychrophile hydrogenase was most active at pH 9.0, and the mesophile hydrogenase was most active at pH 10.0 or higher.